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cell cycle staining buffer  (Multi Sciences (Lianke) Biotech Co Ltd)


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    Structured Review

    Multi Sciences (Lianke) Biotech Co Ltd cell cycle staining buffer
    Cell Cycle Staining Buffer, supplied by Multi Sciences (Lianke) Biotech Co Ltd, used in various techniques. Bioz Stars score: 98/100, based on 1479 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+buffer+kit/Cell+cycle+staining+Kit/pmc13040135-45-5-12
    Average 98 stars, based on 1479 article reviews
    cell cycle staining buffer - by Bioz Stars, 2026-11
    98/100 stars

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    Related Articles

    Staining:

    Article Title: Evaluating the prognostic value of cuproptosis-related genes and characterizing PDE3B’s role in gastric cancer: integrative bioinformatics analysis and experimental validation
    Article Snippet: .. Add 1 mL of DNA Staining Solution (Liankebio, CCS012, China) to the flow tube, vortex to mix thoroughly, and incubate at room temperature protected from light for 30 minutes. ..

    Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration
    Article Snippet: .. After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark. .. DNA content was acquired on a BD LSRFortessa X-20 flow cytometer (excitation 488 nm), and cell-cycle phases were quantified in FlowJo.

    Article Title: A cascade via CD276/PI3K/SIRT1/E-Cad in overcoming contact inhibition of proliferation in hepatocellular carcinoma cells
    Article Snippet: .. Afterward, cells were stained with Cell Cycle Staining Buffer (cat. no. CCS012; Multi Sciences Biotech) in darkness for 30 min. .. Following that, cells were rinsed with PBS, and data acquisition was carried out using a flow cytometer (Canto II; BD Biosciences).

    Article Title: GSK3β as a potential regulator in AML: A pan-cancer multi-omics analysis
    Article Snippet: .. The cell pellet was washed once with PBS and resuspended in 1 mL of DNA Staining Solution (MultiSciences, CCS012, China) containing 10 μL of Permeabilization Solution. ..

    Article Title: Silencing of MAT2A inhibits gastric cancer cell proliferation, migration, and invasion through activation of the p53 pathway.
    Article Snippet: Objective This study aimed to evaluate the effects of MAT2A silencing on the proliferation, migration, and invasion of gastric cancer (GC) cells and to investigate the underlying molecular mechanisms.. Methods MAT2A expression in GC tissues and adjacent normal tissues was assessed using immunohistochemistry and western blotting.. Two GC cell lines with elevated MAT2A expression underwent gene silencing.

    other:

    Article Title: Novel autophagy-promoted cancer therapy: Discovery of matrine-based selective TBC1D2 inhibitors by drug-target complex purification.
    Article Snippet: Autophagy strategies have seen clinical or preclinical studies underway to combat cancer therapeutic resistance, but the on-target effects in normal tissues necessitate new selective approaches.. Based on the antitumor efficacy and low toxicity of matrine, a traditional Chinese medicine component, we designed and synthesized 24 matrine derivatives with higher in vitro activities.. Among these compounds, A1 demonstrated the highest activity and selectivity against the HeLa cell line, with a half maximal inhibitory concentration of 0.43 μM and a selective index of 3.5.

    Incubation:

    Article Title: Silencing of MAT2A inhibits gastric cancer cell proliferation, migration, and invasion through activation of the p53 pathway.
    Article Snippet: Objective This study aimed to evaluate the effects of MAT2A silencing on the proliferation, migration, and invasion of gastric cancer (GC) cells and to investigate the underlying molecular mechanisms.. Methods MAT2A expression in GC tissues and adjacent normal tissues was assessed using immunohistochemistry and western blotting.. Two GC cell lines with elevated MAT2A expression underwent gene silencing.



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